This lesson is aimed at beginner Antha OS users.
About this tutorial
This lesson contains information about the basic features of the plate prep task. To learn how to edit Plate Layout template files, see the editing plate layouts lesson.
Things you'll need
Before you get started you will need an Antha license so that you have access to Antha OS.
What you'll learn
- What the Plate Prep task view is and what features it offers.
- How to physically prepare an input plate based on the instructions given by the Plate Prep task view
- How to download a plate
- How to upload a plate file
- How to find plates in the inventory
- Understanding the colour-coding
Walkthrough of the Plate Prep Task
The Plate Prep task view is accessed by clicking on the Plate Prep icon in the timeline of the Dashboard View of a selected job.
When you open the Plate Prep task, all of your auto-generated plates and previously uploaded plates are show here. You can see what the plates will look like before and after the workflow is completed. You can also click the "3D" button to see a 3D view of the plate and any riser it may be on.
Editing the Input Plate
If you already have an input plate you wish to use and need to change the automatically generated input plate, you can do so by clicking on the green "Edit Plates" Button. In this view, you can either drag your plate file onto the upload area or you can click the blue text to upload the file from your file browser.
Once you upload your file, you will be prompted to submit a name and barcode for the new plate. After you've filled in this information, click "confirm" to continue. Your newly uploaded plate should be visible on the left. Repeat these upload steps for any additional plates. If you are satisfied with the plates, press the yellow "Resimulate" button to go back to the plate view. You will be prompted to confirm that you want to re-simulate your workflow. This step is important to make sure that the plate you've uploaded is valid and will work with the other components in your custom workflow. Click "Confirm" to continue.
Preparing an Input Plate Based on the Plate Prep Task View
The most useful part of the Plate Prep view is using it as a guide for physically preparing the input plates you'll need for your your job. With the visual cues offered by the Plate Prep view, making up your plate is fast and simple.
Knowing the Contents of a Well
To find out what the contents of your input plate are supposed to be, you can click on individual wells, a single row, or a single column to highlight its contents. By doing so, the volume will appear in the selected well and the content details will be highlighted.
The contents of a plate are colour-coded based on how the liquids are handled by the liquid handling robot.
How to Download a Plate
There are three places where you can download the plate layout file: The Type IIS Construct Assembly Protocol card, the Workflow Information Sidebar (which is shared between the Job Queue and the Dashboard) or within the Plate Prep task of an existing job.
Getting an Existing Plate Layout File from the Dashboard or the Job Queue
You can modify the input plate in the Dashboard or the Job Queue by downloading the auto-generated input plate, editing it, and re-uploading it. Scroll down on the Workflow Details sidebar until you see "Plate files." Click on the input plate (the one with the blue icon). Open the downloaded file and save to a memorable location.